Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection

Abstract Electrostatic samplers have been increasingly studied for sampling of viral and bacterial aerosols, bioaerosol are required to provide concentrated liquid samples with high physical collection biological recovery, which would be critical rapid detection. Here, the effects media protocols on recovery two airborne bacteria ( Pseudomonas fluorescens Micrococcus luteus ) under electrostatic field were investigated using a personal particle concentrator (EPC). Deionized (DI) water with/without sodium dodecyl sulfate (SDS) phosphate buffered saline tested as media. A polystyrene container was mounted onto electrode EPC stable storage vortexing after capture. Many cells found deposited bottom surface submerged in via electrophoresis, among protocols, wet subsequent offered most suspension. Experiments several showed that 0.001–0.01% SDS-DI demonstrated highest rate EPC. These findings valuable detection bioaerosols.